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In broccoli, the prevalent glucosinolates include glucoraphanin (4-methylsulfinylbutyl), sinigrin, progoitrin (2-hydroxy-3-butenyl), gluconapine (3-butenyl), glucobrassicin, neoglucobrassicin (1-methoxy-3-indolylmethyl) and glucoraphanin (the most abundant, comprising over 50% of total glucosinolates, while sinigrin is present in comparatively lower amounts) [54]
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We then performed a gene set enrichment analysis (GSEA) to determine the association between the expression of ACTL6A and signaling pathways in the RNA array results, which indicated significant differences between ACTL6A-KD cells and control cells in multiple metabolism-related pathways, among which GSH metabolism ranked as one of the most highly correlated pathways (Fig
