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The Mast Cell Cascade: Minute-by-Minute Biology Mast cells are the central actors in injection-site reactions

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Following the 24 h incubation, the culture medium and pinealocyte suspension will be removed from the flask (the astrocytes will remain attached to the flask and can also be cultured separately if desired) and centrifuged and resuspended in DMEM to a final concentration of 2 × 10 5 cells/mL [35]

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