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The isolation of the nutrient from liver extracts was enhanced by the development of a microbial bioassay [3], using a bacterial vitamin B 12 auxotroph, and culminated in the generation of purified bright red crystals [4,5], that were shown to contain cobalt [6,7], and which famously made their way into the hands of Dorothy Hodgkin for X-ray diffraction studies [8,9]
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