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hope this answer is helpful B6 overdose resulting in neurological symptoms would require daily B6 doses of 100mg for months to years (or B6 injections)

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The 4 amino acids with either a strongly or weakly acidic chain, pI is the average of the two lowest pKa values The 13 amino acids with a neutral side chain, pI is the average of pKa1 and pKa2 The 3 amino acids with a basic side chain, pI is the average of the two highest pKa values