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In mature sperm, ROS production occurs either in the plasma membrane by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase or in the presence of a nicotinamide adenine dinucleotide (NADH)-dependent oxidoreductase in the inner mitochondrial membrane, which ensures ROS production through electron leakage from the electron transport chain [10]

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When these groups discuss results in the same threads without clarifying baseline expectations, the result is confusion