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To produce the positive control, 20 μM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP)) was added to the cells and incubated for 10 min then stained with TMRE as described above

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Ex vivo staining of ROS with fluorescent dyes Ex vivo staining was performed as previously described (Johnson & Rabinovitch, 2012)