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Tethering of Ipl1, through Sli15 and Bir1, to centromere-bound Ndc10 could compensate for the relatively weak affinity of the Ipl1 motif for the Ndc80c head, and the interaction with the Ndc80c head could then localize the kinase domain close to its targets

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The recommended approach is to aliquot the reconstituted solution into single-use portions before freezing, minimizing repeated thermal cycling of the main research stock