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ZSWIM8 intracellular rescue assay Following generation of ZSWIM8 -knockout reporter K562 cells stably expressing doxycycline-inducible ZSWIM8 transgenes, as described above, about 400,000 cells were plated per well in a six-well plate with medium containing 3 µg ml −1 puromycin and 33.3 ng ml −1 doxycycline (Takara Bio, 631311)

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