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(10)制备Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂： (10) Preparation of Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: 在步骤(9)的Fmoc-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂中，加入脱帽试剂10～50℃反应5～30分钟，用真空泵抽干，重新加入脱帽试剂10～50℃反应10～60分钟，抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，加入用DMF溶解的Fmoc-Gly-OH、DIEA、TBTU/HOBt或HBTU/HOBt或BOP/HOBt的混合物，10～50℃反应10～60分钟，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂树脂； In the Fmoc-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin in step (9), add a decapping reagent and react at 10-50°C for 5-30 minutes , dry it with a vacuum pump, re-add the decapping reagent at 10-50 ° C for 10-60 minutes, drain it, wash it with isopropanol for 3 times, wash it with DMF for 3 times, wash it twice with DMF, add the solution dissolved in DMF Mixture of Fmoc-Gly-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt, react at 10-50°C for 10-60 minutes, dry it with a vacuum pump, wash with isopropanol for 3 times, DMF for 3 times, The DMF that redistilled is washed 2 times, sucks dry, obtains Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin resin
