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fumigatus ∆yap1 AfS77 , to yield ∆egtA ∆ yap1 and complementation of egtA in this double mutant was demonstrated (Supplementary Figure S2)

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To address this, we utilized a nuclease-deficient Cas9 (dCas9a) fused to a potent transcriptional activator (VP64-p65-Rta), which allowed us to overexpress all genes encoding mitochondrial membrane proteins (37)