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The condition is characterized by hyperandrogenism, oligo-ovulation, and polycystic ovaries, with 70% of cases going undetected ( The gene repertoire of the gut microbiota is at least 150 times larger than that of the human host, making it a “virtual endocrine organ.” It communicates with the host in both directions through processes such as energy collection, immunological education, and signaling via secondary metabolites ( Firmicutes and a higher proportion of Bacteroidetes , resulting in a lower F/B ratio ( Fusobacterium, Porphyromonas , and Prevotella , is markedly elevated in patients with PCOS, whereas Lactobacillus and Bifidobacterium , which are involved in butyrate production and barrier maintenance, exhibit synchronous depletion ( Lobetyolin, a polyacetylene glycoside extracted from the roots of Codonopsis pilosula , is a crucial quality indicator of this herb ( Codonopsis pilosula inhibits α-glucosidase, with lobetyolin being the principal active constituent ( 2 Materials and methods 2.1 Major instruments and equipment The following equipment was utilized in the study: a high-speed refrigerated centrifuge (Sigma, Germany), ultra-low temperature freezer (Thermo Fisher Scientific, Shanghai, China), paraffin microtome (Leica Biosystems Nussloch GmbH, Germany), thermal cycler (Analytik Jena AG, Germany), automated nucleic acid purification system (Qiagen, Germany), microscopic image analysis system (Olympus Corporation, Japan), and blood glucose meter (Shanghai Yuyan Scientific Instruments Co., Ltd., China)