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Indeed, NanoBRET target engagement assay in live HEK293T cells showed that the irreversible (acrylamide) JNK-IN-8 and the reversible (cyclohexenone) 1a R -IN-8 performed similarly (~ 10 nM), while reversible cyanoacrylamide-based CA-IN-8 was a weaker JNK binder ( ~ 200 nM)
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