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For molecular analyses (Western blot, RT-qPCR, copper measurement, JC-1 assay), brains were rapidly dissected on ice to isolate the SN and striatum, which were then snap-frozen in liquid nitrogen and stored at −80 °C until use

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However, several human studies have indicated that a deficiency in micronutrients, such as zinc, can be associated with insulin resistance as well as greater glucose intolerance