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For digestion, 40-50 μL of the reaction mixture was combined with 10 μL 0.2% (w/v) RapiGest SF (Waters, Milford, USA) prepared in 50 mM ammonium bicarbonate buffer (pH = 7.8) Subsequently, 3.3 μL of 45 mM DTT in 100 mM ammonium bicarbonate buffer was added to the mixture and incubated at 37 °C for 30 min
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Peptide Peptide Benefits & Features Peptides are essential in fundamental physiological processes and for many biochemical processes
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