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The DNA region spanning from − 2 569 pb to + 310 pb of CCL11 (GRCh38/hg38 chr17: 34283173–34286052) was amplified by PCR from bisulfite-converted DNA using a primer set designed to account for cytosine conversion to uracil (Bisulfite Primer Design Tool, Zymo Research) (Additional file 2) and GoTaq DNA Polymerase (Promega, Madison, Wisconsin)
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Input: First test: 200 mIU/mL Second test: 290 mIU/mL Time gap: Exactly 48 hours The calculator checks your percentage increase against the 35 percent minimum baseline
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