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USA 117 , 752–760 (2020)

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A series of 2D 1 H- 15 N HSQC spectra were collected at protein-to-ligand molar ratio of 1:0, 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:5, 1:7, 1:10, 1:15, 1:20, 1:30, 1:40, 1:50 and 1:60 for GlcNAc and 1:0, 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:4, 1:5, 1:6, 1:8, 1:10, 1:15 and 1:20 for GlcNAcβ1-2Man