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As a transcription factor, p53 is responsible for the expression of many genes, including genes associated with apoptotic cell death [194]
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1 × 10 4 293T cells were seeded in each well of a 24-well plate overnight, following which they were co-transfected with wild-type and mutant AQP4 promoter-luciferase plasmids (0.1 μg per well) and 0.4μg miR-146a-3p mimics or NCs using Lipofectamine 2000 (Invitrogen, United States)
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