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The cells were washed twice with DPBS Ca 2+ /Mg 2+ , and fresh RPMI phenol red-free media was added before live cell imaging

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In contrast, DIO mice in both groups switched from WD to LD each had significantly increased Proteobacteria relative abundance (represented solely by the genus Parasutterella ) compared to both the WD and the LD controls, suggesting that Proteobacteria relative abundance may be largely influenced by stress or the differences in net energy expenditure (or associated body weight changes) caused by the diet switch