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A volume of 250 μl of 50% bead slurry were then added and incubated for 3 h rotating at 4 °C

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Assays for HSV-1 protein expression and HSV-1 VP5 exposure A549-GST and A549-MxB cells were inoculated with HSV-1 strain F at the indicated MOI in 2% FBS medium for 60 min on ice in the presence or absence of 100 µg/ml cycloheximide (Sigma-Aldrich)