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Mitochondria then determine the mode of cell death through mechanisms such as apoptosis, autophagy, and ferroptosis, ultimately leading to melanocyte destruction and loss

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Measurement of cytokines in the liver and spleen Liver and spleen tissues homogenized with protein extraction solution (PRO-PREP, iNtRON Biotechnology, Seoul, Korea) and measured the quantity of IL-2, IFN- γ and TNF- α in total proteins (1 mg) using mouse cytokine assay kit (R&D Systems, Minneapolis, MN, USA)