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If we consider the CPYC or CPFC active sites found in glutaredoxins as a reference, all plant Cys-GSTs display a reminiscent catalytic motif that differs by only one residue, with the exception of most GHRs which have two variations in their CPWA motif (Table 2)

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The monothiol mechanism results in a reduced target protein, and a disulfide between the Grx and GSH