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Significant used in this context refers to an amount that would lead to the physiological consequence of accelerated blood clearance of all or part of a second dose with respect to the level of blood clearance expected for a second dose of an ABC triggering LNP

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We firstly validated the ability of both clones to overexpress Nkx2.1 and Pax8 after Dox treatment on the seventh day of the differentiation protocol using immunostaining, showing that the Nrf2 loss-of-function does not affect the tetracycline-inducible overexpression of Nkx2.1 and Pax8